Reporter

Part:BBa_I717001:Design

Designed by: Blair Lyons   Group: iGEM07_Virginia_Tech   (2007-10-03)


reversed dsRED


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 701
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 702
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 716
    Illegal NotI site found at 7
    Illegal NotI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 217
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 702
    Illegal PstI site found at 359
    Illegal PstI site found at 560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 702
    Illegal PstI site found at 359
    Illegal PstI site found at 560
    Illegal PstI site found at 716
    Illegal NgoMIV site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We switched and flipped the prefix and suffix in order to invert the gene.


Source

The original dsRED sequence is from a commercial gene.

References